RanBP1 Antibody

• Catalog Number: orb19210
• Category: Antibodies
• Description: RanBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human RanBP1 recombinant protein (Position: A2-Q201). Human RanBP1 shares 93.1% amino acid (aa) sequence identity with mouse RanBP1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23310 MW
• UniProt ID: P43487
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ran-specific GTPase-activating protein;Ran-binding
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-RanBP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-RanBP1 antibody.Lane 1:human K562 cell;2:human HEK293 cell;3:human HL-60 cell;4:human U20S cell;5:human A431 cell;6:human HEPG2 cell;7:human U937 cell;8:rat testis tissue;9:rat C6 cell;10:mouse testis tissue.

IF analysis of RanBP1 using anti-RanBP1 antibody. RanBP1 was detected in immunocytochemical section of A431 cells.

IHC analysis of RanBP1 using anti-RanBP1 antibody. RanBP1 was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of RanBP1 using anti-RanBP1 antibody. RanBP1 was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of RanBP1 using anti-RanBP1 antibody. RanBP1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of RanBP1 using anti-RanBP1 antibody. RanBP1 was detected in paraffin-embedded section of human lung cancer tissues.