Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody

• Catalog Number: orb1710832
• Category: Antibodies
• Description: Thrombomodulin Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR253L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 17-515 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 500 µg/ml
• Dilution range: WB: 1:1,000, IP: 12 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Thrombomodulin
• UniProt ID: P07204
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CD141 antigen; AHUS6; TM; thrombomodulin; THRM; fe
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Thrombomodulin by western blot. Samples: Whole cell lysate (50 µg) from A-549, MDA-MB-231, A-431 (25 µg) , THP-1, and CAL-27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human lymph node. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-431 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832) used for IP at 12 µl/mg lysate.

Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human SW954 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human A-431 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710832). Secondary: HRP-conjugated goat anti-rabbit IgG.