Rabbit anti-SPTLC1 Antibody

• Catalog Number: orb1524895
• Category: Antibodies
• Description: SPTLC1 Antibody
• Target: SPTLC1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 50 and 100
• UniProt ID: O15269
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: HSAN1; serine palmitoyltransferase 1; SPT1; SPTI;
• Note: For research use only
• NCBI: NP_006406.1

Detection of human and mouse SPTLC1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SPTLC1 antibody orb1524895 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. SPTLC1 was also immunoprecipitated by rabbit anti-SPTLC1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).