Rabbit anti-SPTLC1 Antibody

• Catalog Number: orb1524893
• Category: Antibodies
• Description: SPTLC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 423 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SPTLC1
• UniProt ID: O15269
• NCBI: NP_006406.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HSAN1; serine palmitoyltransferase 1; SPT1; SPTI;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SPTLC1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , and Jurkat (J; 50 µg) cells. Antibody: Affinity purified rabbit anti-SPTLC1 antibody orb1524893 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SPTLC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTLC1 antibody orb1524893 used for IP at 6 µg/mg lysate.