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Catalog Number | orb1526903 |
---|---|
Category | Antibodies |
Description | hSET1B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | Between 350 and 400 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. A302-280A has not performed satisfactorily when used for WB of hSet1B in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of hSet1B.; IP - 2-10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | hSET1B |
UniProt ID | Q9UPS6 |
NCBI | NP_055863.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | lysine N-methyltransferase 2G; SET domain-containi Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human hSET1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1B antibody (orb1526903) used for IP at 6 µg per reaction. hSET1B was also immunoprecipitated by a previous lot of this antibody (orb1526903) and a second antibody against a different epitope of hSET1B.