Rabbit anti-GAPDH Antibody

• Catalog Number: orb1529868
• Category: Antibodies
• Description: GAPDH Antibody
• Target: GAPDH
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 200 µg/ml
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 175 and 225
• UniProt ID: P04406
• Tested applications: IHC, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: GAPD; peptidyl-cysteine S-nitrosylase GAPDH; Oct1
• Note: For research use only
• NCBI: NP_002037.2

Detection of mouse GAPDH by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-GAPDH antibody at 0.04 µg/ml.

Detection of human GAPDH by western blot.Samples: Whole cell lysate from HEK293T, Jurkat, Hep-G2, HeLa, and MCF-7 cells. Antibody: Affinity purified rabbit anti-GAPDH antibody at 0.04 µg/ml.

Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH used at a dilution of 1:1, 000 (0.2µg/ml).

Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-GAPDH used at a dilution of 1:1, 000 (0.2µg/ml).