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Rabbit GAPDH Antibody
SKU: orb1529869
Description
Research Area
Cancer Biology, Metabolism Research, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Feline, Gallus, Golden Hamster, Guinea pig, Porcine, Rabbit, Rat, Sheep, Zebrafish |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 175 and 225 |
| Target | GAPDH |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3PD; GAPD; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; peptidyl-cysteine S-nitrosylase GAPDH
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Detection of human GAPDH by western blot.Samples: Whole cell lysate from HEK293T, Jurkat, Hep-G2, HeLa, and MCF-7 cells. Antibody: Affinity purified rabbit anti-GAPDH antibody at 0.04 µg/ml.
Detection of mouse GAPDH by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-GAPDH antibody at 0.04 µg/ml.
Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-GAPDH used at a dilution of 1:1, 000 (0.2µg/ml).
Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH used at a dilution of 1:1, 000 (0.2µg/ml).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002037.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Rabbit GAPDH Antibody (orb1529869)
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