Rabbit anti-G3BP2 Antibody

SKU: orb1527340

Description

G3BP2 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 275 and 325
• Target: G3BP2

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

G3BP-2; GAP SH3 domain-binding protein 2; GTPase activating protein (SH3 domain) binding protein 2; ras GTPase-activating protein-binding protein 2; Ras-GTPase activating protein SH3 domain-binding protein 2

Detection of human G3BP2 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP2 antibody (orb1527340) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human G3BP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G3BP2 antibody (orb1527340) used for IP at 6 µg per reaction. G3BP2 was also immunoprecipitated by a previous lot of this antibody (orb1527340) and a second antibody against a different epitope of G3BP2.

Quick Database Links

Gene Symbol

G3BP2

UniProt

Q9UN86

RefSeq (Protein):NP_036429.2