Rabbit anti-EZH2 Antibody

• Catalog Number: orb1523445
• Category: Antibodies
• Description: EZH2 Antibody
• Target: EZH2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 696 and C-terminus
• UniProt ID: Q15910
• Tested applications: IP, WB
• Dilution range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: ENX1; KMT6A; WVS2; WVS; lysine N-methyltransferase
• Note: For research use only
• NCBI: CAA64955.1

Detection of mouse EZH2 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EZH2 antibody (orb1523445) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human EZH2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EZH2 antibody (orb1523445) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human EZH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EZH2 antibody (orb1523445) used for IP at 6 µg per reaction. EZH2 was also immunoprecipitated by a previous lot of this antibody (orb1523445) and a second antibody against a different epitope of EZH2.