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Rabbit anti-eIF5B/IF2 Antibody
Catalog Number: orb1527866
| Catalog Number | orb1527866 |
|---|---|
| Category | Antibodies |
| Description | eIF5B/IF2 Antibody |
| Target | eIF5B/IF2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 450 and 500 |
| UniProt ID | O60841 |
| Tested applications | WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-eIF5B/IF2 antibody A301-744A. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | eukaryotic translation initiation factor 5B; IF2; Read more... |
| Note | For research use only |
| NCBI | NP_056988.3 |
Detection of human eIF5B/IF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF5B/IF2 antibody orb1527866 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF5B/IF2 was immunoprecipitated successfully when using rabbit anti-eIF5B/IF2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
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