Rabbit anti-eIF4G3/eIF4GII Antibody

• Catalog Number: orb1527826
• Category: Antibodies
• Description: eIF4G3/eIF4GII Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 300 and 350
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eIF4G3/eIF4GII
• UniProt ID: O43432
• NCBI: NP_003751.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: eIF-4-gamma II; eukaryotic translation initiation
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human eIF4G3/eIF4GII by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, RKO, LNCaP, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody orb1527826 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human eIF4G3/eIF4GII by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4G3/eIF4GII antibody orb1527826 used for IP at 6 µg per reaction.