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Rabbit anti-Calpastatin/CAST Antibody
Catalog Number: orb1522314
| Catalog Number | orb1522314 |
|---|---|
| Category | Antibodies |
| Description | Calpastatin/CAST Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 658 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | Calpastatin/CAST |
| UniProt ID | P20810 |
| NCBI | NP_001035908.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | BS-17; calpain inhibitor; calpastatin; PLACK; sper Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human Calpastatin/CAST by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Calpastatin/CAST antibody orb1522314 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human Calpastatin/CAST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calpastatin/CAST antibody orb1522314 used for IP at 6 µg per reaction.
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