Rabbit anti-c-Kit Antibody

• Catalog Number: orb1525173
• Category: Antibodies
• Description: c-Kit Antibody
• Target: c-Kit
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 926 and C-terminus
• UniProt ID: P10721
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: piebald trait protein; proto-oncogene c-Kit; v-kit
• Note: For research use only
• NCBI: NP_000213.1
• Expiration Date: 12 months from date of receipt.

Detection of human c-Kit by western blot and immunoprecipitation. Samples: Whole cell lysate from human erythroleukemia (HEL) cells (50, 15, and 5 µg for WB; 1 mg for IP, 20% of IP loaded and HEK293T (T; 50 µg). Antibodies: Affinity purified rabbit anti-c-Kit antibody orb1525173 used for WB at 0.1 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of human c-Kit by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-c-Kit (Cat. No. orb1525173) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB