Rabbit anti-ASH1 Antibody

• Catalog Number: orb1527858
• Category: Antibodies
• Description: ASH1 Antibody
• Target: ASH1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 2919 and C-terminus
• UniProt ID: Q9NR48
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: huASH1; ASH1; MRD52; lysine N-methyltransferase 2H
• Note: For research use only
• NCBI: CAI12722.1

Detection of mouse ASH1 by western blot. Samples: Whole cell lysate (50 µg) from mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody orb1527858 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ASH1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody orb1527858 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH1 antibody orb1527858 used for IP at 3 µg per reaction.

Detection of human ASH1 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 (Cat. No. orb1527858) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB