Rabbit ASH1 Antibody

SKU: orb1527859

Description

ASH1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 2919 and C-terminus
• Target: ASH1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

absent small and homeotic disks protein 1 homolog; ASH1; ash1 (absent, small, or homeotic)-like; ASH1L1; ASH1-like protein; histone-lysine N-methyltransferase ASH1L; huASH1; KMT2H; lysine N-methyltransferase 2H; MRD52; probable histone-lysine N-methyltransferase ASH1L

Detection of human ASH1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody orb1527859 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse ASH1 by western blot. Samples: Whole cell lysate (50 µg) from mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH1 antibody orb1527859 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH1 antibody orb1527859 used for IP at 3 µg per reaction.

Detection of human ASH1 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 (Cat. No. orb1527859) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ASH1

UniProt

Q9NR48

RefSeq:CAI12722.1