Rabbit anti-AF6 Antibody

SKU: orb1527031

Description

AF6 Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-AF6 antibody A302-199A.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1774 and C-terminus
• Target: AF6

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

l-afadin; protein AF-6; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; MLL-AF6; ALL1-fused gene from chromosome 6 protein; afadin; AF6; adherens junction formation factor; MLLT4

Detection of human AF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF6 antibody orb1527031 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. AF6 was successfully immunoprecipitated by rabbit anti-AF6 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

AF6

UniProt

P55196

RefSeq:BAA32484.1