Rabbit anti-AF6 Antibody

• Catalog Number: orb1527031
• Category: Antibodies
• Description: AF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: Between 1774 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-AF6 antibody A302-199A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: AF6
• UniProt ID: P55196
• NCBI: BAA32484.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: l-afadin; protein AF-6; myeloid/lymphoid or mixed-
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human AF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF6 antibody orb1527031 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. AF6 was successfully immunoprecipitated by rabbit anti-AF6 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).