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Catalog Number | orb402252 |
---|---|
Category | Antibodies |
Description | RAB27A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human RAB27A recombinant protein (Position: L98-K216). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 27 kDa |
UniProt ID | P51159 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Ras-related protein Rab-27A; Rab-27; GTP-binding p Read more... |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of K562 cells using anti-RAB27A antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of RAB27A using anti-RAB27A antibody.Lane 1:human HeLa cell;2:human Jurkat cell;3:human MCF-7 cell;4:human HepG2 cell;5:human A549 cell;6:rat stomach tissue;7:rat thymus tissue;8:mouse thymus tissue;9:mouse NIH3T3 cell.
IHC analysis of RAB27A using anti-RAB27A antibody.RAB27A was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of RAB27A using anti-RAB27A antibody.RAB27A was detected in paraffin-embedded section of human mammary cancer tissue.
IHC analysis of RAB27A using anti-RAB27A antibody.RAB27A was detected in paraffin-embedded section of rat spleen tissue.
IHC analysis of RAB27A using anti-RAB27A antibody.RAB27A was detected in paraffin-embedded section of rat small intestine tissue.
IHC analysis of RAB27A using anti-RAB27A antibody.RAB27A was detected in paraffin-embedded section of mouse spleen tissue.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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