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Catalog Number | orb381996 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to RAB27A. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Immunogen | Recombinant full length protein of human RAB27 |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | RAB27A |
Entrez | 5873, 11891 |
UniProt ID | Q9ERI2, P51159 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | RAB27; Ras-related protein Rab-27A; Rab-27; GTP-bi Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Simon Heidegger,Florian Stritzke,Sarah Dahl,Juliane Dabler-Plenker,Laura Joachim,Dominik Buschmann,Kaiji Fan,Carolin M. Sauer,Nils Ludwig,Christof Winter,Stefan Enssle,Suqi Li,Markus Perl,Andre Gorgens,Tobias Haas,Erik Thiele Orberg,Sascha Gottert,Catherine Wolfel,Thomas Engleitner,Isidro Cortes-Ciriano,Roland Rad,Wolfgang Herr,Bernd Giebel,Jurgen Ruland,Florian Bassermann,Christoph Coch,Gunther Hartmann,and Hendrik Poeck Targeting nucleic acid sensors in tumor cells to reprogram biogenesis and RNA cargo of extracellular vesicles for T cell-mediated cancer immunotherapy Cell Reports Medicine, 4, 101171 (2023)
Western blot analysis of RAB27 expression in Hela (A), U937 (B), Jurkat (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
Immunofluorescent analysis of RAB27 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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