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Catalog Number | orb1274470 |
---|---|
Category | Antibodies |
Description | RAB27A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 25kDa |
Target | RAB27A |
UniProt ID | P51159 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Ras-related protein Rab-27A, Rab-27, GTP-binding p Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of U-937 cells, using RAB27A antibody (orb1274470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (orb1274470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (orb1274470) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using RAB27A Antibody (orb1274470) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF7 cells using RAB27A antibody (orb1274470). Blue: DAPI for nuclear staining.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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