RAB27 antibody

• Catalog Number: orb1473871
• Category: Antibodies
• Description: Mouse monoclonal antibody to RAB27
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human RAB27. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RAB27A
• Entrez: 5873
• UniProt ID: P51159
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: RAB27; Ras-related protein Rab-27A; Rab-27; GTP-bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAB27 expression in K562 (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 27 kD)

Flow cytometric analysis of K562 cells using Anti-RAB27 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.