PTPRC Antibody

• Catalog Number: orb1671648
• Category: Antibodies
• Description: PTPRC Antibody
• Clonality: Recombinant
• Clone Number: OTH-74D4
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising mice with an EBV-transformed B cell line from a patient with MHC class II deficiency.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: PTPRC
• UniProt ID: P08575
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PTPRC, Receptor-type tyrosine-protein phosphatase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Jukat cells with anti-CD45RA (OTH-74D4). Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric r version of OTH-74D4 (orb1671648) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671648- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-CD45RA antibody OTH-74D4. Paraformaldehyde fixed Jurkat cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of OTH-74D4 (orb1671648 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.