PTPRC Antibody

• Catalog Number: orb1274265
• Category: Antibodies
• Description: PTPRC Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PTPRC/1783R
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG, k
• Immunogen: Human B lymphocytes were used as immunogen for this recombinant CD45RB antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)Western blot: 0.5-1 ug/mlDue to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTPRC
• UniProt ID: P08575
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Receptor-type tyrosine-protein phosphatase C, Leuk
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RTPrediluted IHC only format: incubate for 30 min at RT (1)Western blot: 0.5-1 ug/mlDue to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of recombinant CD45RB antibody and FFPE human tonsil (clone PTPRC/1783R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.