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Catalog Number | orb1252709 |
---|---|
Category | Antibodies |
Description | PTPRC Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PD7/26 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Western Blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PTPRC |
UniProt ID | P08575 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Receptor-type tyrosine-protein phosphatase C, Leuk Read more... |
Note | For research use only |
Application notes | Western Blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).
IHC testing of human tonsil (20X) stained with PD7/26 mAb.
Western blot testing of Daudi cell lysate with CD45RB antibody (clone PD7/26). Predicted molecular weight: ~147/180~220kDa (unmodified/glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free CD45RB Antibody (clone PD7/26). Confirmation of Integrity and Purity of the Antibody.
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