PTPRC Antibody

• Catalog Number: orb1252606
• Category: Antibodies
• Description: PTPRC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM125
• Tested applications: FC, IF, IHC
• Reactivity: Human, Marmoset, Primate
• Isotype: IgG2a, kappa
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.25-0.5 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTPRC
• UniProt ID: P08575
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Receptor-type tyrosine-protein phosphatase C, Leuk
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.25-0.5 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of anti-CD45RO antibody and FFPE human lymphoma.