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Catalog Number | orb1252291 |
---|---|
Category | Antibodies |
Description | PTPRC Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PTPRC/1461 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Human B lymphocytes were used as the immunogen for the CD45 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RTDue to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PTPRC |
UniProt ID | P08575 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Receptor-type tyrosine-protein phosphatase C, Leuk Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlImmunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RTDue to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC test of FFPE human tonsil probed with CD45 antibody (clone PTPRC/1461). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free CD45 Antibody (clone PTPRC/1461). Confirmation of Integrity and Purity of the Antibody.
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