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Catalog Number | orb1252078 |
---|---|
Category | Antibodies |
Description | PTPRC Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CDLA45RA-1 |
Tested applications | FC, IF, IHC |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the anti-CD45RA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PTPRC |
UniProt ID | P08575 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Receptor-type tyrosine-protein phosphatase C, Leuk Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the anti-CD45RA antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human tonsil and anti-CD45RA antibody (CDLA45RA-1)
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