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Catalog Number | orb344326 |
---|---|
Category | Antibodies |
Description | Protein A antibody (Texas Red) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | FC, IF |
Isotype | IgG |
Immunogen | Protein A [Staphylococcus aureus] |
Concentration | 1.0 mg/mL |
Dilution range | FC: User Optimized, IF: 1:500 - 1:2,500 |
Form/Appearance | Lyophilized |
Purity | Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum. Cross reactivity against Protein A from other sources is unknown. |
Conjugation | Texas Red |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Protein A Antibody, Texas Red Conjugated Read more... |
Note | For research use only |
Application notes | Anti-Protein A Texas Red Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Protein A Reduced (Lane1), Protein A Reduced (Lane2), Protein A Non-Reduced (Lane3), Protein A Non-Reduced (Lane4), Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane using Protein A antibody (Texas Red)
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