Protein A antibody (Texas Red)

• Catalog Number: orb344386
• Category: Antibodies
• Description: Protein A antibody (Texas Red)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF
• Isotype: IgG
• Immunogen: Protein A [Staphylococcus aureus]
• Concentration: 10.0 mg/mL
• Dilution range: IF: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum. Cross reactivity against Protein A from other sources is unknown.
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Protein A Antibody Texas Red conjugate,
• Note: For research use only
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Protein A Reduced (Lane1), Protein A Reduced (Lane2), Protein A Non-Reduced (Lane3), Protein A Non-Reduced (Lane4), Load: Lane 1 and 3 - 1.0 ug per lane, Lane 2 and 4 - 0.25 ug per lane using Protein A antibody (Texas Red)