IRE1/ERN1 Rabbit Polyclonal Antibody

SKU: orb3117246

Description

The IRE1/ERN1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, and WB applications. This antibody targets the Serine/threonine-protein kinase/endoribonuclease IRE1 in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human IRE1 recombinant protein (Position: R158-L280).
• Target: Serine/threonine-protein kinase/endoribonuclease IRE1
• Molecular Weight: 170 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERN1; hIRE1p; Inositol requiring protein 1; IRE1; Ire1 alpha; IRE1; ERN1; IRE1P; IRE1α

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase/endoribonuclease IRE1

UniProt

O75460