IRE1/ERN1 Rabbit Polyclonal Antibody

SKU: orb413012

Description

The IRE1/ERN1 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, and WB applications. This antibody targets the Serine/threonine-protein kinase/endoribonuclease IRE1 in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human IRE1 recombinant protein (Position: R158-L280).
• Target: Serine/threonine-protein kinase/endoribonuclease IRE1
• Molecular Weight: 170 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERN1; hIRE1p; Inositol requiring protein 1; IRE1; Ire1 alpha; IRE1; ERN1; IRE1P; IRE1α

Quick Database Links

Gene Symbol

Serine/threonine-protein kinase/endoribonuclease IRE1

UniProt

O75460