Recombinant Human ICOS Protein

SKU: orb2994712

Description

Recombinant Human Inducible T-cell Costimulator is produced by our Mammalian expression system and the target gene encoding Glu21-Phe141 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Immunology & Inflammation

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Recombinant Human ICOSLG (C-6His) (Cat#orb2995052) on HIS1K Biosensor, can bind Recombinant Human ICOS (C-Fc) (Cat#orb2994712) with an affinity constant of 9.56 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Fc
• Expression Region: Glu21-Phe141
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 40.9 KDa. Observed: 47 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inducible T-cell costimulator;activation-inducible lymphocyte immunomediatory molecule; CD278; AILIM; CVID1;ICOS;

Quick Database Links

UniProt

Q9Y6W8