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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Recombinant Human ICOSLG (C-6His) (Cat#orb2995052) on HIS1K Biosensor, can bind Recombinant Human ICOS (C-Fc) (Cat#orb2994712) with an affinity constant of 9.56 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Expression Region | Asp19-Ser258 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 27.72 KDa. Observed: 40-70 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ICOS Ligand; B7 Homolog 2; B7-H2; B7-Like Protein Gl50; B7-Related Protein 1; B7RP-1; CD275; ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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