PRODH Antibody

• Catalog Number: orb1262601
• Category: Antibodies
• Description: PRODH Antibody
• Target: Prodh
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.
• UniProt ID: Q9WU79
• MW: 68 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Proline dehydrogenase 1, mitochondrial, Proline ox
• Note: For research use only
• NCBI: Q9WU79

Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Antibody staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: MDA-MB-468 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of mouse Prodh Antibody in mouse liver, kidney tissue lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.