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Catalog Number | orb412587 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PRKAA2 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human AMPK alpha 2 |
Dilution range | WB: 1:500-2000, IF/ICC: 1:50-200 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | PRKAA2 |
Entrez | 78975, 5563, 108079 |
UniProt ID | Q8BRK8, P54646, Q09137 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti-AMPK antibody, anti-AMPK2 antibody, anti-5'-A Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of AMPK alpha 2 expression in SW620 (A), mouse brain (B), rat heart (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)
Immunofluorescent analysis of AMPK alpha 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, IHC-P | |
Bovine, Canine, Equine, Gallus, Porcine | |
Human, Mouse, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Monkey, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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