AMPK alpha 1/2 (phospho-T183/172) antibody

• Catalog Number: orb224175
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AMPK alpha 1/2 (Phospho-T183/172)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/172 of human AMPK alpha 1/2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAA1; PRKAA2
• Entrez: 5563, 108079, 105787, 65248, 78975, 5562
• UniProt ID: Q5EG47, P54646, Q13131, P54645, Q8BRK8, Q09137
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PRKAA1 antibody, anti AMPK1 antibody, anti 5'
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AMPK alpha 1/2 (Phospho-T183/172) expression in Jurkat Adriamycin-treated (A), K562 Adriamycin-treated (B) whole cell lysates. (Predicted band size: 64; 62 kD; Observed band size: 63 kD)

Immunohistochemical analysis of AMPK alpha 1/2 (Phospho-T183/172) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-AMPK alpha 1/2 (Phospho-T183/172) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.