AMPK alpha 1(phospho-Thr172) antibody

• Catalog Number: orb99303
• Category: Tools
• Description: AMPK alpha 1(phospho-Thr172) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat, Sheep
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 (LR(p-T)SC)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 64 kDa
• Target: phospho-AMPK alpha-2 (Thr172)
• UniProt ID: P54646
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phosph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Li, Weilong et al. Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK Exp Ther Med, 22, 1075 (2021)

PubMed: 34447468

FACS Analysis of Glycophorin A and phospho-AMPK alpha 1/2 (Thr172/183) in Red Blood Cells in WT and AMPK alpha 1 knockout mice using Rabbit Anti-GPA Polyclonal Antibody and Rabbit anti-pAMPK alpha1/2 Thr172/183.

IHC-P analysis of Rat testis using phospho-AMPK alpha-2 Thr172 Polyclonal Antibody at 1:400 dilution.

IHC-P analysis of Mouse brain using phospho-AMPK alpha-2 Thr172 Polyclonal Antibody at 1:400 dilution.

Flow cytometry of Rat spleen.Primary Antibody (green line): Rabbit Anti-AMPK alpha-1 antibody; Dilution:2μg /10^6 cells.

Flow cytometry of A431(Black).Primary Antibody (green line): Rabbit Anti-phospho-AMPK alpha-1 (Thr172)antibody; Dilution:3μg /10^6 cells.

Flow cytometry of Mouse spleen(Black).Primary Antibody (green line): Rabbit Anti-phospho-AMPK alpha-1 (Thr172) antibody; Dilution:3μg /10^6 cells.