PPP2R2A Antibody

• Catalog Number: orb1265061
• Category: Antibodies
• Description: PPP2R2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Monkey, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: PPP2R2A
• UniProt ID: P63151
• NCBI: P63151
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Serine/threonine-protein phosphatase 2A 55 kDa reg
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis in ZR-75-1 cell line lysates (35 ug/lane).