PNPLA2 / ATGL

• Catalog Number: orb19789
• Category: Antibodies
• Description: Goat polyclonal antibody to PNPLA2
• Target: PNPLA2 / ATGL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Protein Sequence: PARPHGPEDKDQ
• RRID: AB_10938889
• MW: 55.3
• Tested applications: ELISA, IF
• Dilution range: ELISA: 1:8000, WB: 0.2-0.6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.2-0.6 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PNPLA2 antibody, anti ATGL antibody, anti pat
• Note: For research use only
• Entrez: 57104

Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing lipid droplet and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).