PNPLA2/ATGL Antibody

• Catalog Number: orb1537790
• Category: Antibodies
• Description: PNPLA2/ATGL Antibody
• Target: PNPLA2 / ATGL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Equine, Goat, Hamster, Human, Monkey, Mouse, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_065109.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B2404 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2404 was determined to be 2.5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PNPLA2, Adipose triglyceride lipase, ATGL, IPLA2ze
• Note: For research use only

PNPLA2 / ATGL antibody (0.5 µg/ml) staining of Human Adipose lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.