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Catalog Number | orb20391 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PLA2R1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC |
Reactivity | Canine, Human, Porcine |
Dilution range | ELISA: 1:1000, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 169; 153; 168 |
Target | PLA2R1 |
Entrez | 22925 |
Protein Sequence | EKKKDTPKQHGT |
RRID | AB_10753544 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PLA2R1 antibody, anti phospholipase A2 recept Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 140kDa band observed in Human Ileum lysates (calculated MW of 153kDa according to NP_001007268.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Greater than 90% as determined by SDS-PAGE. | |
17.4 kDa | |
Yeast |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Rabbit | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
19.4 kDa | |
E.coli |
The human full length PLA2R1 protein has a MW of 168.6 kDa | |
Mammalian |