Phosphotyrosine (P-Tyr) Antibody

• Catalog Number: orb2319086
• Category: Antibodies
• Description: Phosphotyrosine (P-Tyr) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PY20
• Tested applications: FC, IF, WB
• Reactivity: All
• Isotype: IgG2b
• Immunogen: Phosphotyrosine conjugated to KLH
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate);Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: Depends upon the phosphorylated target
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: P-Tyr
• Note: For research use only
• Application notes: Positive Control: MCF-7, MDA-231, T47-D cells or breast carcinoma
• Expiration Date: 12 months from date of receipt.