Phosphotyrosine Antibody

• Catalog Number: orb67362
• Category: Antibodies
• Description: Mouse Anti- Phosphotyrosine Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: G104
• Tested applications: ICC, IHC, IP, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC-P (1:100)
• Conjugation: Unconjugated
• Target: Phosphotyrosine
• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY
• Note: For research use only
• Application notes: 1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phosphotyrosine on normal (left) and EGF treated (right) phosphorylated tyrosines using Phosphotyrosine antibody