Phosphotyrosine antibody

• Catalog Number: orb147705
• Category: Antibodies
• Description: Mouse monoclonal to Phosphotyrosine (FITC). Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a proteins activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: G104
• Tested applications: ICC, IHC, IP, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100)
• Conjugation: FITC
• Target: Phosphotyrosine
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: PhosphoTyrosine (pY) antibody, PhosphoTyrosine (pY
• Note: For research use only
• Application notes: 1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.