Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody

• Catalog Number: orb6955
• Category: Antibodies
• Description: Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Equine
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 SE(p-Y)GN
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: PTPN6
• UniProt ID: P29350
• RRID: AB_10935803
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SHP-1(phospho-Tyr536); PTPN6(phospho Y536); SHP1(p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PTPN6 (Tyr564) / AF647 Conjugated antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-PTPN6 (Tyr564)) Polyclonal Antibody, Unconjugated (orb6955) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A431 Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-PTPN6 (Tyr564) (orb6955) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65kD, Observed band size: 65kD.

Sample: A549 Cell (Human) Lysate at 40 ug, MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-PTPN6 (Tyr564) (orb6955) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.