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Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody (BF647)
Catalog Number: orb1579395
| Catalog Number | orb1579395 |
|---|---|
| Category | Antibodies |
| Description | Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody (BF647) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IF |
| Predicted Reactivity | Canine, Equine |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 SE(p-Y)GN |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | Flow-Cyt=2ug/test, IF=1:100-500 |
| Conjugation | BF647 |
| Target | PTPN6 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | SHP-1(Phospho-Tyr536); PTPN6(phospho Y536); SHP1(p Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PTPN6 (Tyr564)/AF647 Conjugated antibody (orb1579395), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG- AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
