Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody (BF647)

• Catalog Number: orb1579395
• Category: Antibodies
• Description: Phospho-PTPN6 (Tyr536) Rabbit Polyclonal Antibody (BF647)
• Target: PTPN6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF647
• Reactivity: Human
• Predicted Reactivity: Canine, Equine
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr536 SE(p-Y)GN
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=2ug/test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: SHP-1(phospho-Tyr536); PTPN6(phospho Y536); SHP1(p
• Research Area: Developmental Biology, G Protein-Coupled Receptors
• Note: For research use only

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PTPN6 (Tyr564)/AF647 Conjugated antibody (orb1579395), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG- AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.