Phospho-PDGFRA (Tyr849)/ PDGFRB (Tyr857) Rabbit Polyclonal Antibody

SKU: orb6660

Description

Phospho-PDGFRA (Tyr849)/ PDGFRB (Tyr857) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 SN(p-Y)VS
• Target: PDGFRA
• Molecular Weight: 117 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Receptor beta(phospho Y857); p-Receptor beta(phospho Y857); PDGF Receptor alpha(phospho Y849); p-PDGF Receptor alpha(phospho Y849); PDGF receptor alpha+beta(phospho Y849+Y857); PDGF receptor alpha+beta(phospho Tyr849+Tyr857); PDGF Receptor alpha(Tyr849)/PDGF Receptor beta(Tyr857); phospho-PDGFRA/PDGFRB(Tyr849/Tyr857); Platelet–dirived growth factor receptor-alpha; alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PGFRA) Polyclonal Antibody, Unconjugated (orb6660) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857)) polyclonal Antibody, Unconjugated (orb6660) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

PDGFRA

UniProt

P16234