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Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Rabbit Polyclonal Antibody (BF488)

SKU: orb1598694

Description

Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Rabbit Polyclonal Antibody (BF488)

Images & Validation

Tested ApplicationsICC, IF
Dilution rangeIF=1:100-500, ICC/IF=1:100-500
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Gallus, Mouse, Rat, Sheep

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 SN(p-Y)VS
TargetPDGFRA
PurificationAffinity purified by Protein A

Storage & Handling

StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Receptor beta(phospho Y857); p-Receptor beta(phospho Y857); PDGF Receptor alpha(phospho Y849); p-PDGF Receptor alpha(phospho Y849); PDGF receptor alpha+beta(phospho Y849+Y857); PDGF receptor alpha+beta(phospho Tyr849+Tyr857); PDGF Receptor alpha(Tyr849)/PDGF Receptor beta(Tyr857); phospho-PDGFRA/PDGFRB(Tyr849/Tyr857); Platelet–dirived growth factor receptor-alpha; alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN.
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Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Rabbit Polyclonal Antibody (BF488)

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-PDGFRA antibody (orb1598694), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

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Protocol Information

IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

Phospho-PDGFRA (Tyr849) / PDGFRB (Tyr857) Rabbit Polyclonal Antibody (BF488) (orb1598694)

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100 μl
$ 540.00
DispatchUsually dispatched within 2-3 weeks
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