Phospho-IRAK4 (Thr345) Rabbit Polyclonal Antibody

• Catalog Number: orb184289
• Category: Antibodies
• Description: Phospho-IRAK4 (Thr345) Rabbit Polyclonal Antibody
• Target: IRAK4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345 VM(p-T)SR
• UniProt ID: Q9NWZ3
• MW: 51 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL-1 Receptor-associated Kinase 4; 8430405M07Rik;
• Note: For research use only

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: Raw264.7-lps (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-IRAK4 (Thr345) (orb184289) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51-55 kD, Observed band size: 58 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-IRAK4 (Thr345) Polyclonal Antibody, Unconjugated (orb184289) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.