Phospho-Histone H2A.X (Ser139) Antibody

• Catalog Number: orb1733000
• Category: Antibodies
• Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2C8
• Tested applications: ICC, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500-1/1000; ICC: 1/50-1/200
• Conjugation: Unconjugated
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Entrez: 3014
• UniProt ID: P16104
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.