Histone H2A.X (phospho-Ser139) antibody

• Catalog Number: orb559106
• Category: Antibodies
• Description: Histone H2A.X (phospho-Ser139) antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 1C14
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human H2AX around the phosphorylation site of Ser139 (QA(p-S)QE)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 16 kDa
• Target: Phospho-H2AX (Ser139)
• UniProt ID: P16104
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Histone H2A.X (S139); Phospho-Histone H2A.X (Ser14
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC-P analysis of mouse stomach using Phospho-Histone H2A.X Ser139 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of rat testis using Phospho-Histone H2A.X Ser139 Monoclonal Antibody at 1:200 dilution.

Western blot:Lane 1:MCF-7 (Human) Cell;Lane 2:293T (Human) Cell;Lane 3:Jurkat (Human) Cell;Lane 4:Hela (Human) Cell;Primary:Anti-Phospho-Histone H2A.X (Ser139) at 1:1000 dilution.